| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ELF5 |
| Gene ID: | 2001 |
| Uniprot ID: | ELF5_HUMAN |
| Immunogen Region: | 40-150 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-150 of human ELF5 (NP_938195.1). |
| Immunogen Sequence: | EEYYPAFEHQTACDSYWTSV HPEYWTKRHVWEWLQFCCDQ YKLDTNCISFCNFNISGLQL CSMTQEEFVEAAGLCGEYLY FILQNIRTQGYSFFNDAEES KATIKDYADSN |
| Tissue Specificity | Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher. Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression. |
| Function | Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGAAT. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters. |
| Protein Name | Ets-Related Transcription Factor Elf-5 E74-Like Factor 5 Epithelium-Restricted Ese-1-Related Ets Factor Epithelium-Specific Ets Transcription Factor 2 Ese-2 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets-Related Transcription Factor Elf-5 antibody Anti-E74-Like Factor 5 antibody Anti-Epithelium-Restricted Ese-1-Related Ets Factor antibody Anti-Epithelium-Specific Ets Transcription Factor 2 antibody Anti-Ese-2 antibody Anti-ELF5 antibody Anti-ESE2 antibody |
Information sourced from Uniprot.org