| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EIF4G2 |
| Gene ID: | 1982 |
| Uniprot ID: | IF4G2_HUMAN |
| Immunogen Region: | 750-850 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 750-850 of human EIF4G2/p97 (NP_001409.3). |
| Immunogen Sequence: | IYKWIKDNISPKLHVDKGFV NILMTSFLQYISSEVNPPSD ETDSSSAPSKEQLEQEKQLL LSFKPVMQKFLHDHVDLQVS ALYALQVHCYNSNFPKGMLL R |
| Tissue Specificity | Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney. |
| Post Translational Modifications | Phosphorylation.hyperphosphorylated during mitosis. |
| Function | Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases. |
| Protein Name | Eukaryotic Translation Initiation Factor 4 Gamma 2Eif-4-Gamma 2Eif-4g 2Eif4g 2Death-Associated Protein 5Dap-5P97 |
| Database Links | Reactome: R-HSA-1169408 |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 4 Gamma 2 antibodyAnti-Eif-4-Gamma 2 antibodyAnti-Eif-4g 2 antibodyAnti-Eif4g 2 antibodyAnti-Death-Associated Protein 5 antibodyAnti-Dap-5 antibodyAnti-P97 antibodyAnti-EIF4G2 antibodyAnti-DAP5 antibodyAnti-OK antibodyAnti-SW-cl.75 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and EIF4G2/p97 knockout (KO) 293T cells, using [KO Validated] EIF4G2/p97 Rabbit polyclonal antibody (STJ11100866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89800/358268/STJ11100866_1__08358.1713122610.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and EIF4G2/p97 knockout (KO) 293T cells, using [KO Validated] EIF4G2/p97 Rabbit polyclonal antibody (STJ11100866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
