Anti-EIF3F antibody (88-357) (STJ29103)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EIF3F (88-357) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF3F |
| Gene ID | 8665 |
| Uniprot ID | EIF3F_HUMAN |
| Immunogen Region | 88-357 |
| Immunogen Sequence | GGRVVRLHPVILASIVDSYE RRNEGAARVIGTLLGTVDKH SVEVTNCFSVPHNESEDEVA VDMEFAKNMYELHKKVSPNE LILGWYATGHDITEHSVLIH EYYSREAPNPIHLTVDTSLQ NGRMSIKAYVSTLMGVPGRT MGVMFTPLTVKYAYYDTERI GVDLIMKTCFSPNRVIGLSS DLQQVGGASARIQDALSTVL QYAEDVLSGKVSADNTVGRF LMSLVNQVPKIVPDDFETM |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 88-357 of human EIF3F (NP_003745.1). |
Additional Info
| Post Translational Modifications | Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11. |
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit FEif3fDeubiquitinating Enzyme Eif3fEukaryotic Translation Initiation Factor 3 Subunit 5Eif-3-EpsilonEif3 P47 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 3 Subunit F antibodyAnti-Eif3f antibodyAnti-Deubiquitinating Enzyme Eif3f antibodyAnti-Eukaryotic Translation Initiation Factor 3 Subunit 5 antibodyAnti-Eif-3-Epsilon antibodyAnti-Eif3 P47 antibodyAnti-EIF3F antibodyAnti-EIF3S5 antibody |
Information sourced from Uniprot.org