Anti-eIF3B antibody [R03-9U1] (STJA0037368)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human eIF3B
General Information
| Short Description | Rabbit monoclonal anti-eIF3B for use in WB, ICC, IF, IP and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R03-9U1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | EIF3B |
| Gene ID | 8662 |
| Uniprot ID | EIF3B_HUMAN |
| Immunogen | A synthesized peptide derived from human eIF3B |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation is enhanced upon serum stimulation. |
| Function | RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit BEif3bEukaryotic Translation Initiation Factor 3 Subunit 9Prt1 HomologHprt1Eif-3-EtaEif3 P110Eif3 P116 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | CytoplasmStress GranuleLocalizes To Stress Granules Following Cellular Stress |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 3 Subunit B antibodyAnti-Eif3b antibodyAnti-Eukaryotic Translation Initiation Factor 3 Subunit 9 antibodyAnti-Prt1 Homolog antibodyAnti-Hprt1 antibodyAnti-Eif-3-Eta antibodyAnti-Eif3 P110 antibodyAnti-Eif3 P116 antibodyAnti-EIF3B antibodyAnti-EIF3S9 antibody |
Information sourced from Uniprot.org