• Western blot analysis of extracts of various cell lines, using EIF2B1 antibody (STJ110201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using EIF2B1 antibody (STJ110201) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using EIF2B1 antibody (STJ110201) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using EIF2B1 antibody (STJ110201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF2B1 antibody (1-305) (STJ110201)

SKU:
STJ110201

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF2B1 (1-305) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B1
Gene ID: 1967
Uniprot ID: EI2BA_HUMAN
Immunogen Region: 1-305
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human EIF2B1 (NP_001405.1).
Immunogen Sequence: MDDKELIEYFKSQMKEDPDM ASAVAAIRTLLEFLKRDKGE TIQGLRANLTSAIETLCGVD SSVAVSSGGELFLRFISLAS LEYSDYSKCKKIMIERGELF LRRISLSRNKIADLCHTFIK DGATILTHAYSRVVLRVLEA AVAAKKRFSVYVTESQPDLS GKKMAKALCHLNVPVTVVLD AAVGYIMEKADLVIVGAEGV VENGGIINKIGTNQMAVCAK AQNKPFYVVAESFKFVRLF
Function Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Protein Name Translation Initiation Factor Eif-2b Subunit Alpha
Eif-2b Gdp-Gtp Exchange Factor Subunit Alpha
Database Links Reactome: R-HSA-72731
Alternative Antibody Names Anti-Translation Initiation Factor Eif-2b Subunit Alpha antibody
Anti-Eif-2b Gdp-Gtp Exchange Factor Subunit Alpha antibody
Anti-EIF2B1 antibody
Anti-EIF2BA antibody

Information sourced from Uniprot.org

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