Western blot analysis of extracts of various cell lines, using eIF2A antibody (STJ118645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF2A antibody (1-100) (STJ118645)

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STJ118645
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2A
Gene ID: 83939
Uniprot ID: EIF2A_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2A (NP_114414.2).
Immunogen Sequence: MAPSTPLLTVRGSEGLYMVN GPPHFTESTVFPRESGKNCK VCIFSKDGTLFAWGNGEKVN IISVTNKGLLHSFDLLKAVC LEFSPKNTVLATWQPYTTSK
Tissue Specificity Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
Function Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.
Protein Name Eukaryotic Translation Initiation Factor 2a
Eif-2a
65 Kda Eukaryotic Translation Initiation Factor 2a Cleaved Into - Eukaryotic Translation Initiation Factor 2a - N-Terminally Processed
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 2a antibody
Anti-Eif-2a antibody
Anti-65 Kda Eukaryotic Translation Initiation Factor 2a Cleaved Into - Eukaryotic Translation Initiation Factor 2a - N-Terminally Processed antibody
Anti-EIF2A antibody
Anti-CDA02 antibody
Anti-MSTP004 antibody
Anti-MSTP089 antibody

Information sourced from Uniprot.org

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