Western blot analysis of extracts of various cell lines, using eIF2A antibody (STJ118645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF2A antibody (1-100) (STJ118645)

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STJ118645
£110.50 - £505.50
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Applications:WB/ELISA
Host:Rabbit
Reactivity:Human/Mouse
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:EIF2A
Gene ID:83939
Uniprot ID:EIF2A_HUMAN
Immunogen Region:1-100
Immunogen Sequence:MAPSTPLLTVRGSEGLYMVN GPPHFTESTVFPRESGKNCK VCIFSKDGTLFAWGNGEKVN IISVTNKGLLHSFDLLKAVC LEFSPKNTVLATWQPYTTSK
Specificity:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2A (NP_114414.2).
Tissue Specificity Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
Function Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.
Protein Name Eukaryotic Translation Initiation Factor 2a
Eif-2a
65 Kda Eukaryotic Translation Initiation Factor 2a Cleaved Into - Eukaryotic Translation Initiation Factor 2a - N-Terminally Processed
Database Links
Cellular Localisation
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 2a antibody
Anti-Eif-2a antibody
Anti-65 Kda Eukaryotic Translation Initiation Factor 2a Cleaved Into - Eukaryotic Translation Initiation Factor 2a - N-Terminally Processed antibody
Anti-EIF2A antibody
Anti-CDA02 antibody
Anti-MSTP004 antibody
Anti-MSTP089 antibody

Information sourced from Uniprot.org

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