• Western blot analysis of the lysates from HepG2 cells using EHHADH antibody.
  • Western blot analysis of lysates from A549 cells, using EHHADH Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of A549 cells using PBFE Polyclonal Antibody
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EHHADH Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PBFE Polyclonal Antibody

Anti-EHHADH antibody (476-525 aa) (STJ94975)

SKU:
STJ94975

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Peroxisomal bifunctional enzyme (476-525 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EHHADH
Gene ID: 1962
Uniprot ID: ECHP_HUMAN
Immunogen Region: 476-525 aa
Specificity: PBFE Polyclonal Antibody detects endogenous levels of PBFE protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human EHHADH at the amino acid range 476-525
Post Translational Modifications Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
Function Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway. Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity. Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways. Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation.
Protein Name Peroxisomal Bifunctional Enzyme
Pbe
Pbfe
L-Bifunctional Protein
Lbp
Multifunctional Enzyme 1
Mfe1 Includes - Enoyl-Coa Hydratase/3 -2-Trans-Enoyl-Coa Isomerase - 3-Hydroxyacyl-Coa Dehydrogenase
Database Links Reactome: R-HSA-390247
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-Peroxisomal Bifunctional Enzyme antibody
Anti-Pbe antibody
Anti-Pbfe antibody
Anti-L-Bifunctional Protein antibody
Anti-Lbp antibody
Anti-Multifunctional Enzyme 1 antibody
Anti-Mfe1 Includes - Enoyl-Coa Hydratase/3 -2-Trans-Enoyl-Coa Isomerase - 3-Hydroxyacyl-Coa Dehydrogenase antibody
Anti-EHHADH antibody
Anti-ECHD antibody

Information sourced from Uniprot.org

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