| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EHHADH (444-723) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EHHADH |
| Gene ID: |
1962 |
| Uniprot ID: |
ECHP_HUMAN |
| Immunogen Region: |
444-723 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 444-723 of human EHHADH (NP_001957.2). |
| Immunogen Sequence: |
EVIPSQYSSPTTIATVMNLS KKIKKIGVVVGNCFGFVGNR MLNPYYNQAYFLLEEGSKPE EVDQVLEEFGFKMGPFRVSD LAGLDVGWKSRKGQGLTGPT LLPGTPARKRGNRRYCPIPD VLCELGRFGQKTGKGWYQYD KPLGRIHKPDPWLSKFLSRY RKTHHIEPRTISQDEILERC LYSLINEAFRILGEGIAASP EHIDVVYLHGYGWPRHKGGP MFYASTVGLPTVLEKLQKY |
| Tissue Specificity | Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain. |
| Post Translational Modifications | Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. |
| Function | Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway. Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity. Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways. Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation. |
| Protein Name | Peroxisomal Bifunctional EnzymePbePbfeL-Bifunctional ProteinLbpMultifunctional Enzyme 1Mfe1 Includes - Enoyl-Coa Hydratase/3 -2-Trans-Enoyl-Coa Isomerase - 3-Hydroxyacyl-Coa Dehydrogenase |
| Database Links | Reactome: R-HSA-390247Reactome: R-HSA-9033241 |
| Cellular Localisation | Peroxisome |
| Alternative Antibody Names | Anti-Peroxisomal Bifunctional Enzyme antibodyAnti-Pbe antibodyAnti-Pbfe antibodyAnti-L-Bifunctional Protein antibodyAnti-Lbp antibodyAnti-Multifunctional Enzyme 1 antibodyAnti-Mfe1 Includes - Enoyl-Coa Hydratase/3 -2-Trans-Enoyl-Coa Isomerase - 3-Hydroxyacyl-Coa Dehydrogenase antibodyAnti-EHHADH antibodyAnti-ECHD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
