| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EHD1 |
| Gene ID: | 10938 |
| Uniprot ID: | EHD1_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EHD1 (Q9H4M9). |
| Immunogen Sequence: | MFSWVSKDARRKKEPELFQT VAEGLRQLYAQKLLPLEEHY RFHEFHSPALEDADFDNKPM VLLVGQYSTGKTTFIRHLIE QDFPGMRIGPEPTTDSFIAV |
| Tissue Specificity | Highly expressed in testis. |
| Function | ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis. |
| Protein Name | Eh Domain-Containing Protein 1 Past Homolog 1 Hpast1 Testilin |
| Database Links | Reactome: R-HSA-983231 |
| Cellular Localisation | Recycling Endosome Membrane Peripheral Membrane Protein Cytoplasmic Side Early Endosome Membrane Cell Membrane Cell Projection Cilium Membrane Preferentially Associates With Tubular Recycling Endosomes Colocalizes With Fer1l5 At Plasma Membrane In Myoblasts And Myotubes Localizes To The Ciliary Pocket From Where The Cilium Protrudes Colocalizes With Bace1 In Tubulovesicular Cytoplasmic Membranes Colocalizes With Bace1 And App Amyloid Beta Proteins In Hippocampal Mossy Fiber Terminals |
| Alternative Antibody Names | Anti-Eh Domain-Containing Protein 1 antibody Anti-Past Homolog 1 antibody Anti-Hpast1 antibody Anti-Testilin antibody Anti-EHD1 antibody Anti-PAST antibody Anti-PAST1 antibody Anti-CDABP0131 antibody |
Information sourced from Uniprot.org