| Host: | Mouse |
| Applications: | WB/IHC-P/ICC/IF/ELISA |
| Reactivity: | Vertebrates |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-EGFR-L858Q Mutant for use in WB, IHC-P, ICC, IF and ELISA in Vertebrates samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in PBS (without Mg2+ and Ca2+) , pH 7.4, 150 mM NaCl, 50% glycerol |
| Purification: | Purified from ascites |
| Dilution Range: | WB-1:500-1:1000IHC-P-1:50-1:100ELISA-1:1000-1:5000IF-1:50-1:200 |
| Storage Instruction: | Store at-20°C. Avoid freeze/thaw cycles. |
| Immunogen: | A synthetic peptide from the internal region of EGFR which includes the mutation of L858Q, human origin. |
| Background | EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
Information sourced from Uniprot.org