| Host: | Mouse |
| Applications: | ELISA/WB |
| Reactivity: | eGFP |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-EGFP (1-239aa) for use in ELISA and WB in eGFP samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | AT1D9 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2ak |
| Formulation: | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Purification: | By protein-G affinity chromatography |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | 1-239aa |
| Accession Number: | ABW34377 |
| Immunogen: | Recombinant EGFP (1-239aa) purified from E. coli |
| Background | Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids. |
Information sourced from Uniprot.org
![Anti-eGFP antibody [SM5111] (STJA0005111)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/180165/403566/STJA0005111_1__19373.1738950587.png?c=1 "Anti-eGFP antibody [SM5111] (STJA0005111)")