| Host: | Rabbit |
| Applications: | ELISA/WB/ICC/IP |
| Reactivity: | Chicken/Human/Mouse/Rat/Xenopus |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-eEF2K (C-Term) for use in ELISA, WB, ICC and IP in Chicken, Human, Mouse, Rat and Xenopus samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000ICC 1:200IP 1:100 |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | EEF2K |
| Gene ID: | 29904 |
| Uniprot ID: | EF2K_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human A431, HeLa, and Jurkat cells. The antibody also detected eEF2K in immunoprecipitations in HeLa cells and immunofluorescent labeling |
| Immunogen: | eEF2K (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human eEF2K. This sequence is well conserved in rat, mouse, chicken, and fish eEF2K, and has low homology to other proteins. |
| Immunogen Sequence: | Human |
| Post Translational Modifications | Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis. |
| Function | Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. |
| Protein Name | Eukaryotic Elongation Factor 2 KinaseEef-2 KinaseEef-2kCalcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase |
| Database Links | Reactome: R-HSA-166208 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Eukaryotic Elongation Factor 2 Kinase antibodyAnti-Eef-2 Kinase antibodyAnti-Eef-2k antibodyAnti-Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase antibodyAnti-EEF2K antibody |
Information sourced from Uniprot.org