Western blot analysis of various lysates using eEF1G Rabbit pAb (STJ113571) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-EEF1G antibody (168-437) (STJ113571)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ113571
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General Information

Short DescriptionRabbit polyclonal EEF1G (168-437) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolEEF1G
Gene ID1937
Uniprot IDEF1G_HUMAN
Immunogen Region168-437
Immunogen SequenceLLWLYKQVLEPSFRQAFPNT NRWFLTCINQPQFRAVLGEV KLCEKMAQFDAKKFAETQPK KDTPRKEKGSREEKQKPQAE RKEEKKAAAPAPEEEMDECE QALAAEPKAKDPFAHLPKST FVLDEFKRKYSNEDTLSVAL PYFWEHFDKDGWSLWYSEYR FPEELTQTFMSCNLITGMFQ RLDKLRKNAFASVILFGTNN SSSISGVWVFRGQELAFPLS PDWQVDYESYTWRKLDPGS
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human eEF1G (NP_001395.1).

Additional Info

Tissue Specificity Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
Function Probably plays a role in anchoring the complex to other cellular components.
Protein Name Elongation Factor 1-Gamma
Ef-1-Gamma
Eef-1b Gamma
Database Links Reactome: R-HSA-156842
Reactome: R-HSA-9725370
Cellular Localisation
Alternative Antibody Names Anti-Elongation Factor 1-Gamma antibody
Anti-Ef-1-Gamma antibody
Anti-Eef-1b Gamma antibody
Anti-EEF1G antibody
Anti-EF1G antibody
Anti-PRO1608 antibody

Information sourced from Uniprot.org

Citations

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