• Western blot analysis of extracts of various cell lines, using eEF1G antibody (STJ113571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of NIH-3T3 cells using eEF1G Polyclonal Antibody (STJ113571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat brain using eEF1G rabbit polyclonal antibody (STJ113571) at dilution of 1:50 (40x lens).
  • Immunofluorescence analysis of C6 cells using eEF1G Polyclonal Antibody (STJ113571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human breast cancer using eEF1G rabbit polyclonal antibody (STJ113571) at dilution of 1:50 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using eEF1G Rabbit polyclonal antibody (STJ113571) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using eEF1G rabbit polyclonal antibody (STJ113571) at dilution of 1:50 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using eEF1G Rabbit polyclonal antibody (STJ113571) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using eEF1G Polyclonal Antibody (STJ113571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using eEF1G Rabbit polyclonal antibody (STJ113571) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using eEF1G Polyclonal Antibody (STJ113571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using eEF1G antibody (STJ113571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-EEF1G antibody (168-437) (STJ113571)

SKU:
STJ113571

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EEF1G (168-437) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF1G
Gene ID: 1937
Uniprot ID: EF1G_HUMAN
Immunogen Region: 168-437
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human eEF1G (NP_001395.1).
Immunogen Sequence: LLWLYKQVLEPSFRQAFPNT NRWFLTCINQPQFRAVLGEV KLCEKMAQFDAKKFAETQPK KDTPRKEKGSREEKQKPQAE RKEEKKAAAPAPEEEMDECE QALAAEPKAKDPFAHLPKST FVLDEFKRKYSNEDTLSVAL PYFWEHFDKDGWSLWYSEYR FPEELTQTFMSCNLITGMFQ RLDKLRKNAFASVILFGTNN SSSISGVWVFRGQELAFPLS PDWQVDYESYTWRKLDPGS
Tissue Specificity Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
Function Probably plays a role in anchoring the complex to other cellular components.
Protein Name Elongation Factor 1-Gamma
Ef-1-Gamma
Eef-1b Gamma
Database Links Reactome: R-HSA-156842
Reactome: R-HSA-9725370
Alternative Antibody Names Anti-Elongation Factor 1-Gamma antibody
Anti-Ef-1-Gamma antibody
Anti-Eef-1b Gamma antibody
Anti-EEF1G antibody
Anti-EF1G antibody
Anti-PRO1608 antibody

Information sourced from Uniprot.org

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