• Western blot analysis of extracts from normal (control) and ECE1 knockout (KO) HeLa cells, using ECE1 antibody (STJ27605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry of paraffin-embedded rat spleen using ECE1 antibody (STJ27605) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human mammary cancer using ECE1 antibody (STJ27605) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse kidney using ECE1 antibody (STJ27605) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of L929 cells using ECE1 Polyclonal Antibody (STJ27605) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ECE1 antibody (670-770) (STJ27605)

SKU:
STJ27605

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECE1 (670-770) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECE1
Gene ID: 1889
Uniprot ID: ECE1_HUMAN
Immunogen Region: 670-770
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 670-770 of human ECE1 (NP_001388.1).
Immunogen Sequence: ADNGGLKAAYRAYQNWVKKN GAEHSLPTLGLTNNQLFFLG FAQVWCSVRTPESSHEGLIT DPHSPSRFRVIGSLSNSKEF SEHFRCPPGSPMNPPHKCEV W
Tissue Specificity All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma.isoforms A and C in liver, testis and small intestine.isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells.isoforms C and D in saphenous vein cells, and isoform C in kidney.
Function Converts big endothelin-1 to endothelin-1.
Protein Name Endothelin-Converting Enzyme 1
Ece-1
Database Links Reactome: R-HSA-375276
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Endothelin-Converting Enzyme 1 antibody
Anti-Ece-1 antibody
Anti-ECE1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance