• Immunofluorescence analysis of L929 cells using ECE1 Rabbit polyclonal antibody (STJ11104569) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ECE1 in paraffin-embedded mouse kidney using ECE1 Rabbit polyclonal antibody (STJ11104569) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ECE1 in paraffin-embedded human mammary cancer using ECE1 Rabbit polyclonal antibody (STJ11104569) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ECE1 in paraffin-embedded rat spleen using ECE1 Rabbit polyclonal antibody (STJ11104569) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells using ECE1 Rabbit polyclonal antibody (STJ11104569) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:180s.

Anti-ECE1 antibody (670-770) (STJ11104569)

SKU:
STJ11104569

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Endothelin-Converting Enzyme 1 (670-770) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECE1
Gene ID: 1889
Uniprot ID: ECE1_HUMAN
Immunogen Region: 670-770
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 670-770 of human ECE1 (NP_001388.1).
Immunogen Sequence: ADNGGLKAAYRAYQNWVKKN GAEHSLPTLGLTNNQLFFLG FAQVWCSVRTPESSHEGLIT DPHSPSRFRVIGSLSNSKEF SEHFRCPPGSPMNPPHKCEV W
Tissue Specificity All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma.isoforms A and C in liver, testis and small intestine.isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells.isoforms C and D in saphenous vein cells, and isoform C in kidney.
Function Converts big endothelin-1 to endothelin-1.
Protein Name Endothelin-Converting Enzyme 1
Ece-1
Database Links Reactome: R-HSA-375276
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Endothelin-Converting Enzyme 1 antibody
Anti-Ece-1 antibody
Anti-ECE1 antibody

Information sourced from Uniprot.org

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