| Host: | Mouse |
| Applications: | WB/IF/IP |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Transcription factor E2F1 is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: | Monoclonal |
| Clone ID: | 4G8-2C2-C12 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500ICC 1:100IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | E2F1 |
| Gene ID: | 1869 |
| Uniprot ID: | E2F1_HUMAN |
| Specificity: | This antibody detects endogenous levels of E2F-1 and does not cross-react with related proteins. |
| Immunogen: | Purified recombinant human E2F-1 protein fragments expressed in E.coli. |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Directly activates transcription of PEG10. Positively regulates transcription of RRP1B. |
| Protein Name | Transcription Factor E2f1E2f-1Pbr3Retinoblastoma-Associated Protein 1Rbap-1Retinoblastoma-Binding Protein 3Rbbp-3Prb-Binding Protein E2f-1 |
| Database Links | Reactome: R-HSA-111448Reactome: R-HSA-113501Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-139915Reactome: R-HSA-1912408Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-6804116Reactome: R-HSA-68911Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69656Reactome: R-HSA-8953750Reactome: R-HSA-9616222Reactome: R-HSA-9661069 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2f1 antibodyAnti-E2f-1 antibodyAnti-Pbr3 antibodyAnti-Retinoblastoma-Associated Protein 1 antibodyAnti-Rbap-1 antibodyAnti-Retinoblastoma-Binding Protein 3 antibodyAnti-Rbbp-3 antibodyAnti-Prb-Binding Protein E2f-1 antibodyAnti-E2F1 antibodyAnti-RBBP3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
