| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-E2F1 (380-430) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.65 µg/µl |
| Dilution Range: | WB: 1:1, 000 DB: 1:10, 000 ELISA: 1:10, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | E2F1 |
| Gene ID: | 1869 |
| Uniprot ID: | E2F1_HUMAN |
| Immunogen Region: | 380-430 |
| Immunogen: | Synthetic peptide taken within amino acid region 380-430 on E2F1 protein. |
| Post Translational Modifications | Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. Phosphorylation at Ser-403 by GSK3B promotes interaction with USP11, leading to its deubiquitination and stabilization. Ubiquitinated via 'Lys-63'-linked ubiquitin, leading to its degradation. Deubiquitinated by USP11 following phosphorylation by GSK3B, promoting its stability. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. Methylation at Lys-185 by SETD7 promotes E2F1 ubiquitin-dependent proteasomal degradation. |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Directly activates transcription of PEG10. Positively regulates transcription of RRP1B. |
| Protein Name | Transcription Factor E2f1 E2f-1 Pbr3 Retinoblastoma-Associated Protein 1 Rbap-1 Retinoblastoma-Binding Protein 3 Rbbp-3 Prb-Binding Protein E2f-1 |
| Database Links | Reactome: R-HSA-111448 Reactome: R-HSA-113501 Reactome: R-HSA-1362277 Reactome: R-HSA-1362300 Reactome: R-HSA-139915 Reactome: R-HSA-1912408 Reactome: R-HSA-2559580 Reactome: R-HSA-2559585 Reactome: R-HSA-6804116 Reactome: R-HSA-68911 Reactome: R-HSA-69202 Reactome: R-HSA-69205 Reactome: R-HSA-69231 Reactome: R-HSA-69656 Reactome: R-HSA-8953750 Reactome: R-HSA-9616222 Reactome: R-HSA-9661069 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2f1 antibody Anti-E2f-1 antibody Anti-Pbr3 antibody Anti-Retinoblastoma-Associated Protein 1 antibody Anti-Rbap-1 antibody Anti-Retinoblastoma-Binding Protein 3 antibody Anti-Rbbp-3 antibody Anti-Prb-Binding Protein E2f-1 antibody Anti-E2F1 antibody Anti-RBBP3 antibody |
Information sourced from Uniprot.org