Anti-E1 Ubiquitin Activating Enzyme antibody [R01-6T7] (STJA0036846)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human E1 Ubiquitin Activating Enzyme
General Information
| Short Description | Rabbit monoclonal anti-E1 Ubiquitin Activating Enzyme for use in WB, IHC-P, ICC, IF and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-6T7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | UBA1 |
| Gene ID | 7317 |
| Uniprot ID | UBA1_HUMAN |
| Immunogen | A synthesized peptide derived from human E1 Ubiquitin Activating Enzyme |
Additional Info
| Tissue Specificity | Detected in erythrocytes (at protein level). Ubiquitous. |
| Post Translational Modifications | ISGylated. |
| Function | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme 1Protein A1s9Ubiquitin-Activating Enzyme E1 |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmMitochondrionNucleusIsoform 1: NucleusIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Like Modifier-Activating Enzyme 1 antibodyAnti-Protein A1s9 antibodyAnti-Ubiquitin-Activating Enzyme E1 antibodyAnti-UBA1 antibodyAnti-A1S9T antibodyAnti-UBE1 antibody |
Information sourced from Uniprot.org