Anti-DROSHA antibody (774-823 aa) (STJ97614)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RNase III Drosha at the amino acid range 774-823
General Information
| Short Description | Rabbit polyclonal anti-Ribonuclease 3 (774-823 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DROSHA |
| Gene ID | 29102 |
| Uniprot ID | RNC_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RNase III Drosha at the amino acid range 774-823 |
| Immunogen Region | 774-823 aa |
| Specificity | RNase III Drosha Polyclonal Antibody detects endogenous levels of RNase III Drosha protein. |
Additional Info
| Post Translational Modifications | Degraded by autophagy in response to neuronal activity in motor neurons. |
| Function | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons. |
| Protein Name | Ribonuclease 3Protein DroshaRibonuclease IiiRnase IiiP241 |
| Database Links | Reactome: R-HSA-203927 |
| Cellular Localisation | NucleusNucleolusCytoplasmA Fraction Is Translocated To The Nucleolus During The S Phase Of The Cell CycleLocalized In Gw Bodies (Gwbs)Also Known As P-Bodies |
| Alternative Antibody Names | Anti-Ribonuclease 3 antibodyAnti-Protein Drosha antibodyAnti-Ribonuclease Iii antibodyAnti-Rnase Iii antibodyAnti-P241 antibodyAnti-DROSHA antibodyAnti-RN3 antibodyAnti-RNASE3L antibodyAnti-RNASEN antibody |
Information sourced from Uniprot.org