• Western blot analysis of lysates from brain tissue, using RNase III Drosha antibody.
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Western blot analysis of SH-SY5Y K562 HELA using RNase III Drosha antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-DROSHA antibody (774-823 aa) (STJ97614)

SKU:
STJ97614

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ribonuclease 3 (774-823 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DROSHA
Gene ID: 29102
Uniprot ID: RNC_HUMAN
Immunogen Region: 774-823 aa
Specificity: RNase III Drosha Polyclonal Antibody detects endogenous levels of RNase III Drosha protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RNase III Drosha at the amino acid range 774-823
Function Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
Protein Name Ribonuclease 3
Protein Drosha
Ribonuclease Iii
Rnase Iii
P241
Database Links Reactome: R-HSA-203927
Cellular Localisation Nucleus
Nucleolus
A Fraction Is Translocated To The Nucleolus During The S Phase Of The Cell Cycle
Localized In Gw Bodies (Gwbs)
Also Known As P-Bodies
Alternative Antibody Names Anti-Ribonuclease 3 antibody
Anti-Protein Drosha antibody
Anti-Ribonuclease Iii antibody
Anti-Rnase Iii antibody
Anti-P241 antibody
Anti-DROSHA antibody
Anti-RN3 antibody
Anti-RNASE3L antibody
Anti-RNASEN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance