STJ73722 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Doublecortin/DCX antibody (69-81) (STJ73722)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ73722-100
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General Information

Short DescriptionGoat polyclonal antibody anti-Doublecortin/DCX (69-81) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/IHC/FC
HostGoat
ReactivityHuman/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6ยตg/ml.
IF: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolDCX
Gene ID1641
Uniprot IDDCX_HUMAN
Accession NumberNP_000546.2
Immunogen Region69-81
Immunogen SequenceDRSHCQSLRFHQN
SpecificityThis antibody is expected to recognize reported isoform a (NP_000546.2) only.

Additional Info

Post Translational Modifications Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules. Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21. Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.
Function Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Protein Name Neuronal Migration Protein Doublecortin
Doublin
Lissencephalin-X
Lis-X
Database Links Reactome: R-HSA-447043
Cellular Localisation Cytoplasm
Cell Projection
Neuron Projection
Localizes At Neurite Tips
Alternative Antibody Names Anti-Neuronal Migration Protein Doublecortin antibody
Anti-Doublin antibody
Anti-Lissencephalin-X antibody
Anti-Lis-X antibody
Anti-DCX antibody
Anti-DBCN antibody
Anti-LISX antibody

Information sourced from Uniprot.org

Citations

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