Anti-Doublecortin antibody (Full-Length) [30] (STJA0040768)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenFull length recombinant human Lis-A isoform of Doublecortin purified from E. coli.
General Information
| Short Description | Mouse monoclonal anti-Doublecortin (Full-Length) for use in ICC and WB in Bovine, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Mouse |
| Reactivity | Bovine/Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 30 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 1:500-1:2000ICC 1:500-1:1000 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 ยตL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20ยฐC for |
Target Information
| Gene Symbol | DCX |
| Gene ID | 1641 |
| Uniprot ID | DCX_HUMAN |
| Immunogen | Full length recombinant human Lis-A isoform of Doublecortin purified from E. coli. |
| Immunogen Region | Full-Length |
| Immunogen Sequence | Human |
| Specificity | The antibody reacts with two bands at ~45 kDa and ~35 kDa which shows that the mouse anti-DCX antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A. It has also been use |
Additional Info
| Tissue Specificity | Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. |
| Post Translational Modifications | Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules. Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21. Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells. |
| Function | Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. |
| Protein Name | Neuronal Migration Protein DoublecortinDoublinLissencephalin-XLis-X |
| Database Links | Reactome: R-HSA-447043 |
| Cellular Localisation | CytoplasmCell ProjectionNeuron ProjectionLocalizes At Neurite Tips |
| Alternative Antibody Names | Anti-Neuronal Migration Protein Doublecortin antibodyAnti-Doublin antibodyAnti-Lissencephalin-X antibodyAnti-Lis-X antibodyAnti-DCX antibodyAnti-DBCN antibodyAnti-LISX antibody |
Information sourced from Uniprot.org