• Western blot analysis of extracts of various cell lines, using DOK4 antibody (STJ23423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DOK4 rabbit polyclonal antibody (STJ23423) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using DOK4 rabbit polyclonal antibody (STJ23423) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using DOK4 rabbit polyclonal antibody (STJ23423) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using DOK4 rabbit polyclonal antibody (STJ23423) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-DOK4 antibody (1-326) (STJ23423)

SKU:
STJ23423

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DOK4 (1-326) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DOK4
Gene ID: 55715
Uniprot ID: DOK4_HUMAN
Immunogen Region: 1-326
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human DOK4 (NP_060580.2).
Immunogen Sequence: MATNFSDIVKQGYVKMKSRK LGIYRRCWLVFRKSSSKGPQ RLEKYPDEKSVCLRGCPKVT EISNVKCVTRLPKETKRQAV AIIFTDDSARTFTCDSELEA EEWYKTLSVECLGSRLNDIS LGEPDLLAPGVQCEQTDRFN VFLLPCPNLDVYGECKLQIT HENIYLWDIHNPRVKLVSWP LCSLRRYGRDATRFTFEAGR MCDAGEGLYTFQTQEGEQIY QRVHSATLAIAEQHKRVLL
Tissue Specificity Widely expressed. High expression in skeletal muscle, heart, kidney and liver. Weaker expression in spleen, lung and small intestine, brain, heart and. Expressed in both resting and activated peripheral blood T-cells.
Post Translational Modifications Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.
Function DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.
Protein Name Docking Protein 4
Downstream Of Tyrosine Kinase 4
Insulin Receptor Substrate 5
Irs-5
Irs5
Database Links Reactome: R-HSA-8853659
Alternative Antibody Names Anti-Docking Protein 4 antibody
Anti-Downstream Of Tyrosine Kinase 4 antibody
Anti-Insulin Receptor Substrate 5 antibody
Anti-Irs-5 antibody
Anti-Irs5 antibody
Anti-DOK4 antibody

Information sourced from Uniprot.org

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