| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Dedicator of cytokinesis protein 8 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DOCK8 |
| Gene ID: |
81704 |
| Uniprot ID: |
DOCK8_HUMAN |
| Specificity: |
This antibody detects endogenous levels of DOCK8 at Human, Mouse |
| Immunogen: |
Synthesized peptide derived from human DOCK8 |
| Post Translational Modifications | In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1. |
| Function | Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC. Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing. |
| Protein Name | Dedicator Of Cytokinesis Protein 8 |
| Database Links | Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013409Reactome: R-HSA-983231 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionLamellipodium MembraneEnriched And Co-Localizes With Gtpase Cdc42 At The Immunological Synapse Formed During T Cell/Antigen Presenting Cell Cognate InteractionTranslocates From The Cytoplasm To The Plasma Membrane In Response To Chemokine Cxcl12/Sdf-1-Alpha Stimulation |
| Alternative Antibody Names | Anti-Dedicator Of Cytokinesis Protein 8 antibodyAnti-DOCK8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
