Anti-DOCK8 antibody (1900-2099 aa) (STJ11107480)

SKU:
STJ11107480

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Gene Symbol: DOCK8
Gene ID: 81704
Uniprot ID: DOCK8_HUMAN
Immunogen Region: 1900-2099 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1900-2099 of human DOCK8 (NP_982272.2).
Immunogen Sequence: TLEGRPRGELHEQYRRNTVL TTMHAFPYIKTRISVIQKEE FVLTPIEVAIEDMKKKTLQL AVAINQEPPDAKMLQMVLQG SVGATVNQGPLEVAQVFLAE IPADPKLYRHHNKLRLCFKE FIMRCGEAVEKNKRLITADQ REYQQELKKNYNKLKENLRP MIERKIPELYKPIFRVESQK RDSFHRSSFRKCETQLSQGS
Tissue Specificity Expressed in peripheral blood mononuclear cells (PBMCs).
Post Translational Modifications In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1.
Function Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC. Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing.
Protein Name Dedicator Of Cytokinesis Protein 8
Database Links Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013409
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Lamellipodium Membrane
Enriched And Co-Localizes With Gtpase Cdc42 At The Immunological Synapse Formed During T Cell/Antigen Presenting Cell Cognate Interaction
Translocates From The Cytoplasm To The Plasma Membrane In Response To Chemokine Cxcl12/Sdf-1-Alpha Stimulation
Alternative Antibody Names Anti-Dedicator Of Cytokinesis Protein 8 antibody
Anti-DOCK8 antibody

Information sourced from Uniprot.org