| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DNMT3L (151-386) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNMT3L |
| Gene ID: |
29947 |
| Uniprot ID: |
DNM3L_HUMAN |
| Immunogen Region: |
151-386 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 151-386 of human DNMT3L (NP_787063.1). |
| Immunogen Sequence: |
SGLLQRRRKWRSQLKAFYDR ESENPLEMFETVPVWRRQPV RVLSLFEDIKKELTSLGFLE SGSDPGQLKHVVDVTDTVRK DVEEWGPFDLVYGATPPLGH TCDRPPSWYLFQFHRLLQYA RPKPGSPRPFFWMFVDNLVL NKEDLDVASRFLEMEPVTIP DVHGGSLQNAVRVWSNIPAI RSRHWALVSEEELSLLAQNK QSSKLAAKWPTKLVKNCFLP LREYFKYFSTELTSSL |
| Tissue Specificity | Expressed at low levels in several tissues including testis, ovary, and thymus. |
| Function | Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression. |
| Protein Name | DnaCytosine-5-Methyltransferase 3-Like |
| Database Links | Reactome: R-HSA-5334118 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna antibodyAnti-Cytosine-5-Methyltransferase 3-Like antibodyAnti-DNMT3L antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
