• Immunofluorescence analysis of NIH/3T3 cells using DNASE1L1 Rabbit polyclonal antibody (STJ115116) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using DNASE1L1 Rabbit polyclonal antibody (STJ115116) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from mouse heart, using DNASE1L1 Rabbit polyclonal antibody (STJ115116) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-DNASE1L1 antibody (1-302) (STJ115116)

SKU:
STJ115116

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNASE1L1
Gene ID: 1774
Uniprot ID: DNSL1_HUMAN
Immunogen Region: 1-302
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human DNASE1L1 (NP_006721.1).
Immunogen Sequence: MHYPTALLFLILANGAQAFR ICAFNAQRLTLAKVAREQVM DTLVRILARCDIMVLQEVVD SSGSAIPLLLRELNRFDGSG PYSTLSSPQLGRSTYMETYV YFYRSHKTQVLSSYVYNDED DVFAREPFVAQFSLPSNVLP SLVLVPLHTTPKAVEKELNA LYDVFLEVSQHWQSKDVILL GDFNADCASLTKKRLDKLEL RTEPGFHWVIADGEDTTVRA STHCTYDRVVLHGERCRSL
Tissue Specificity Highest levels in skeletal and cardiac muscles. Detectable in all other tissues tested except brain.
Protein Name Deoxyribonuclease-1-Like 1
Dnase X
Deoxyribonuclease I-Like 1
Dnase I-Like 1
Muscle-Specific Dnase I-Like
Xib
Database Links Reactome: R-HSA-6798695
Cellular Localisation Endoplasmic Reticulum
Alternative Antibody Names Anti-Deoxyribonuclease-1-Like 1 antibody
Anti-Dnase X antibody
Anti-Deoxyribonuclease I-Like 1 antibody
Anti-Dnase I-Like 1 antibody
Anti-Muscle-Specific Dnase I-Like antibody
Anti-Xib antibody
Anti-DNASE1L1 antibody
Anti-DNAS1L1 antibody
Anti-DNL1L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance