Anti-Dnajc5 antibody (177-191) (STJ72913)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Dnajc5 (177-191) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 20kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.1kDa according to NP_058055.1). Recommended concentration: 0.5-1.5ยตg/ml. Preliminary testing was uns |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | DNAJC5 |
| Gene ID | 80331 |
| Uniprot ID | DNJC5_HUMAN |
| Accession Number | NP_058055.1 |
| Immunogen Region | 177-191 |
| Immunogen Sequence | SATETTQLTADSHPS |
Additional Info
| Post Translational Modifications | Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58. This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions. Palmitoylated. Could be palmitoylated by DHHC3, DHHC7, DHHC15 and DHHC17. Palmitoylation occurs probably in the cysteine-rich domain and regulates DNAJC5 membrane attachment. |
| Function | Acts as a general chaperone in regulated exocytosis. Acts as a co-chaperone for the SNARE protein SNAP-25. Involved in the calcium-mediated control of a late stage of exocytosis. May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings. |
| Protein Name | Dnaj Homolog Subfamily C Member 5Ceroid-Lipofuscinosis Neuronal Protein 4Cysteine String ProteinCsp |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-888590Reactome: R-HSA-9662360 |
| Cellular Localisation | CytoplasmCytosolMembraneLipid-AnchorCytoplasmic VesicleSecretory VesicleChromaffin Granule MembraneMelanosomeCell MembraneThe Association With Membranes Is Regulated By PalmitoylationIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily C Member 5 antibodyAnti-Ceroid-Lipofuscinosis Neuronal Protein 4 antibodyAnti-Cysteine String Protein antibodyAnti-Csp antibodyAnti-DNAJC5 antibodyAnti-CLN4 antibody |
Information sourced from Uniprot.org