| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-DNA replication ATP-dependent helicase/nuclease DNA2 is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DNA2 |
| Gene ID: | 1763 |
| Uniprot ID: | DNA2_HUMAN |
| Specificity: | DNA2 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from part of the human protein |
| Function | Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function. |
| Protein Name | Dna Replication Atp-Dependent Helicase/Nuclease Dna2 Hdna2 Dna Replication Atp-Dependent Helicase-Like Homolog Includes - Dna Replication Nuclease Dna2 - Dna Replication Atp-Dependent Helicase Dna2 |
| Database Links | Reactome: R-HSA-174437 Reactome: R-HSA-5685938 Reactome: R-HSA-5685942 Reactome: R-HSA-5693554 Reactome: R-HSA-5693568 Reactome: R-HSA-5693579 Reactome: R-HSA-5693607 Reactome: R-HSA-5693616 Reactome: R-HSA-6804756 Reactome: R-HSA-69166 Reactome: R-HSA-69473 Reactome: R-HSA-9701192 Reactome: R-HSA-9704331 Reactome: R-HSA-9704646 Reactome: R-HSA-9709570 Reactome: R-HSA-9709603 |
| Cellular Localisation | Nucleus Mitochondrion Was Initially Reported To Be Exclusively Mitochondrial However It Was Later Shown To Localize Both In Mitochondrion And Nucleus |
| Alternative Antibody Names | Anti-Dna Replication Atp-Dependent Helicase/Nuclease Dna2 antibody Anti-Hdna2 antibody Anti-Dna Replication Atp-Dependent Helicase-Like Homolog Includes - Dna Replication Nuclease Dna2 - Dna Replication Atp-Dependent Helicase Dna2 antibody Anti-DNA2 antibody Anti-DNA2L antibody Anti-KIAA0083 antibody |
Information sourced from Uniprot.org