Anti-DMTN antibody (356-405 aa) (STJ92690)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Dematin at the amino acid range 356-405
General Information
| Short Description | Rabbit polyclonal anti-Dematin (356-405 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DMTN |
| Gene ID | 2039 |
| Uniprot ID | DEMA_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Dematin at the amino acid range 356-405 |
| Immunogen Region | 356-405 aa |
| Specificity | Dematin Polyclonal Antibody detects endogenous levels of Dematin protein. |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation at Ser-403 by PKA causes the C-terminal headpiece domain to associate with the N-terminal core domain, and leads to the inhibition of its actin bundling activity. The N-terminus is blocked. |
| Function | Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Also plays a role as a modulator of actin dynamics in fibroblasts.acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation. |
| Protein Name | DematinDematin Actin-Binding ProteinErythrocyte Membrane Protein Band 4.9 |
| Database Links | Reactome: R-HSA-5223345 |
| Cellular Localisation | CytoplasmCytosolPerinuclear RegionCytoskeletonCell MembraneMembraneEndomembrane SystemCell ProjectionLocalized At The Spectrin-Actin Junction Of Erythrocyte Plasma MembraneLocalized To Intracellular Membranes And The Cytoskeletal NetworkLocalized At Intracellular Membrane-Bounded Organelle Compartment In Platelets That Likely Represent The Dense Tubular Network MembraneDetected At The Cell Membrane And At The Parasitophorous Vacuole In Malaria-Infected Erythrocytes At Late Stages Of Plasmodium Berghei Or Falciparum Development |
| Alternative Antibody Names | Anti-Dematin antibodyAnti-Dematin Actin-Binding Protein antibodyAnti-Erythrocyte Membrane Protein Band 4.9 antibodyAnti-DMTN antibodyAnti-DMT antibodyAnti-EPB49 antibody |
Information sourced from Uniprot.org