• Immunofluorescence analysis of L929 cells using DLAT antibody (STJ116741) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using DLAT antibody (STJ116741) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using DLAT antibody (STJ116741) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using DLAT antibody (STJ116741) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using DLAT Rabbit polyclonal antibody (STJ116741) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DLAT antibody (87-270) (STJ116741)

SKU:
STJ116741

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DLAT (87-270) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLAT
Gene ID: 1737
Uniprot ID: ODP2_HUMAN
Immunogen Region: 87-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 87-270 of human DLAT (NP_001922.2).
Immunogen Sequence: SLPPHQKVPLPSLSPTMQAG TIARWEKKEGDKINEGDLIA EVETDKATVGFESLEECYMA KILVAEGTRDVPIGAIICIT VGKPEDIEAFKNYTLDSSAA PTPQAAPAPTPAATASPPTP SAQAPGSSYPPHMQVLLPAL SPTMTMGTVQRWEKKVGEKL SEGDLLAEIETDKATIGFEV QEEG
Post Translational Modifications Delipoylated at Lys-132 and Lys-259 by SIRT4, delipoylation decreases the PHD complex activity.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Protein Name Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate Dehydrogenase Complex - Mitochondrial
70 Kda Mitochondrial Autoantigen Of Primary Biliary Cirrhosis
Pbc
Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex
M2 Antigen Complex 70 Kda Subunit
Pyruvate Dehydrogenase Complex Component E2
Pdc-E2
Pdce2
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-389661
Reactome: R-HSA-5362517
Reactome: R-HSA-70268
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate Dehydrogenase Complex - Mitochondrial antibody
Anti-70 Kda Mitochondrial Autoantigen Of Primary Biliary Cirrhosis antibody
Anti-Pbc antibody
Anti-Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex antibody
Anti-M2 Antigen Complex 70 Kda Subunit antibody
Anti-Pyruvate Dehydrogenase Complex Component E2 antibody
Anti-Pdc-E2 antibody
Anti-Pdce2 antibody
Anti-DLAT antibody
Anti-DLTA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance