Western blot analysis of extracts of various cell lines, using DLAT rabbit monoclonal antibody (STJ11102381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-DLAT antibody (548-647) [S1MR] (STJ11102381)

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STJ11102381
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLAT
Gene ID: 1737
Uniprot ID: ODP2_HUMAN
Immunogen Region: 548-647
Specificity: A synthetic peptide corresponding to a sequence within amino acids 548-647 of human DLAT (P10515).
Immunogen Sequence: AREGKLQPHEFQGGTFTISN LGMFGIKNFSAIINPPQACI LAIGASEDKLVPADNEKGFD VASMMSVTLSCDHRVVDGAV GAQWLAEFRKYLEKPITMLL
Post Translational Modifications Delipoylated at Lys-132 and Lys-259 by SIRT4, delipoylation decreases the PHD complex activity.
Function As part of the pyruvate dehydrogenase complex, catalyzes the transfers of an acetyl group to a lipoic acid moiety (Probable). The pyruvate dehydrogenase complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable).
Protein Name Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate Dehydrogenase Complex - Mitochondrial
70 Kda Mitochondrial Autoantigen Of Primary Biliary Cirrhosis
Pbc
Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex
M2 Antigen Complex 70 Kda Subunit
Pyruvate Dehydrogenase Complex Component E2
Pdc-E2
Pdce2
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-5362517
Reactome: R-HSA-9857492
Reactome: R-HSA-9861559
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate Dehydrogenase Complex - Mitochondrial antibody
Anti-70 Kda Mitochondrial Autoantigen Of Primary Biliary Cirrhosis antibody
Anti-Pbc antibody
Anti-Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex antibody
Anti-M2 Antigen Complex 70 Kda Subunit antibody
Anti-Pyruvate Dehydrogenase Complex Component E2 antibody
Anti-Pdc-E2 antibody
Anti-Pdce2 antibody
Anti-DLAT antibody
Anti-DLTA antibody

Information sourced from Uniprot.org

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