• Western blot analysis of extracts of various cell lines, using DIRAS3 antibody (STJ110259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using DIRAS3 antibody (STJ110259) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DIRAS3 antibody (1-229) (STJ110259)

SKU:
STJ110259

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DIRAS3 (1-229) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DIRAS3
Gene ID: 9077
Uniprot ID: DIRA3_HUMAN
Immunogen Region: 1-229
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human DIRAS3 (NP_004666.1).
Immunogen Sequence: MGNASFGSKEQKLLKRLRLL PALLILRAFKPHRKIRDYRV VVVGTAGVGKSTLLHKWASG NFRHEYLPTIENTYCQLLGC SHGVLSLHITDSKSGDGNRA LQRHVIARGHAFVLVYSVTK KETLEELKAFYELICKIKGN NLHKFPIVLVGNKSDDTHRE VALNDGATCAMEWNCAFMEI SAKTDVNVQELFHMLLNYKK KPTTGLQEPEKKSQMPNTTE KLLDKCIIM
Tissue Specificity Expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers.
Protein Name Gtp-Binding Protein Di-Ras3
Distinct Subgroup Of The Ras Family Member 3
Rho-Related Gtp-Binding Protein Rhoi
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Antibody Names Anti-Gtp-Binding Protein Di-Ras3 antibody
Anti-Distinct Subgroup Of The Ras Family Member 3 antibody
Anti-Rho-Related Gtp-Binding Protein Rhoi antibody
Anti-DIRAS3 antibody
Anti-ARHI antibody
Anti-NOEY2 antibody
Anti-RHOI antibody

Information sourced from Uniprot.org

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