• Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3-K27 antibody (STJ23988) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K27 Rabbit polyclonal antibody (STJ23988) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DiMethyl-Histone H3-K27 in paraffin-embedded rat lung tissue using DiMethyl-Histone H3-K27 Rabbit polyclonal antibody (STJ23988) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DiMethyl-Histone H3-K27 in paraffin-embedded mouse spleen tissue using DiMethyl-Histone H3-K27 Rabbit polyclonal antibody (STJ23988) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DiMethyl-Histone H3-K27 in paraffin-embedded human colon tissue using DiMethyl-Histone H3-K27 Rabbit polyclonal antibody (STJ23988) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody (STJ23988) at 1:1000 dilution.
  • Western blot analysis of various lysates, using DiMethyl-Histone H3-K27 Rabbit polyclonal antibody (STJ23988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Di-Methyl-Histone H3-K27 antibody (16-37) (STJ23988)

SKU:
STJ23988

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Histone H3-K27 (16-37) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:100
ChIP-seq 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 16-37
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 16-37 of human histone H3 (NP_003520.1).
Immunogen Sequence: APRKQLATKAARKSAPATGG VK
Background Enables damaged DNA binding activity and single-stranded DNA binding activity. Involved in DNA repair and DNA replication. Part of DNA replication factor A complex.

Information sourced from Uniprot.org

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