Anti-DHX58 antibody (389-678) (STJ110556)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DHX58 (389-678) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DHX58 |
| Gene ID | 79132 |
| Uniprot ID | DHX58_HUMAN |
| Immunogen Region | 389-678 |
| Immunogen Sequence | QQQGLQTVDIRAQLLIGAGN SSQSTHMTQRDQQEVIQKFQ DGTLNLLVATSVAEEGLDIP HCNVVVRYGLLTNEISMVQA RGRARADQSVYAFVATEGSR ELKRELINEALETLMEQAVA AVQKMDQAEYQAKIRDLQQA ALTKRAAQAAQRENQRQQFP VEHVQLLCINCMVAVGHGSD LRKVEGTHHVNVNPNFSNYY NVSRDPVVINKVFKDWKPGG VISCRNCGEVWGLQMIYKS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 389-678 of human DHX58 (NP_077024.2). |
Additional Info
| Tissue Specificity | Expressed in testis, nerve and spleen. Also expressed in the brain. |
| Function | Acts as a regulator of RIGI and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to RIGI and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on RIG-I signaling may involve the following mechanisms: competition with RIGI for binding to the viral RNA, binding to RIGI and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by RIGI and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses and coronavirus SARS-CoV-2, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. |
| Protein Name | Atp-Dependent Rna Helicase Dhx58Atp-Dependent Helicase Lgp2Protein D11lgp2 HomologRig-I-Like Receptor 3Rlr-3Rig-I-Like Receptor Lgp2Rlr |
| Database Links | Reactome: R-HSA-168928 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Atp-Dependent Rna Helicase Dhx58 antibodyAnti-Atp-Dependent Helicase Lgp2 antibodyAnti-Protein D11lgp2 Homolog antibodyAnti-Rig-I-Like Receptor 3 antibodyAnti-Rlr-3 antibodyAnti-Rig-I-Like Receptor Lgp2 antibodyAnti-Rlr antibodyAnti-DHX58 antibodyAnti-D11LGP2E antibodyAnti-LGP2 antibody |
Information sourced from Uniprot.org