• Immunofluorescence analysis of U2OS cells using DHRS9 antibody (STJ28246). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DHRS9 antibody (STJ28246) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using DHRS9 antibody (STJ28246) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Basic Kit. _Exposure time: 390s.

Anti-DHRS9 antibody (18-319) (STJ28246)

SKU:
STJ28246

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DHRS9 (18-319) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DHRS9
Gene ID: 10170
Uniprot ID: DHRS9_HUMAN
Immunogen Region: 18-319
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1).
Immunogen Sequence: RKGKLKIEDITDKYIFITGC DSGFGNLAARTFDKKGFHVI AACLTESGSTALKAETSERL RTVLLDVTDPENVKRTAQWV KNQVGEKGLWGLINNAGVPG VLAPTDWLTLEDYREPIEVN LFGLISVTLNMLPLVKKAQG RVINVSSVGGRLAIVGGGYT PSKYAVEGFNDSLRRDMKAF GVHVSCIEPGLFKTNLADPV KVIEKKLAIWEQLSPDIKQQ YGEGYIEKSLDKLKGNKSY
Tissue Specificity Highly expressed in trachea and epidermis. Detected at lower levels in spinal cord, bone marrow, brain, tongue, esophagus, heart, colon, testis, placenta, lung, skeletal muscle and lymph node.
Function 3-alpha-hydroxysteroid dehydrogenase that converts 3-alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. Also plays a role in the biosynthesis of retinoic acid from retinaldehyde. Can utilize both NADH and NADPH.
Protein Name Dehydrogenase/Reductase Sdr Family Member 9
3-Alpha Hydroxysteroid Dehydrogenase
3-Alpha-Hsd
Nadp-Dependent Retinol Dehydrogenase/Reductase
Rdh-E2
Rdhl
Retinol Dehydrogenase 15
Short Chain Dehydrogenase/Reductase Family 9c Member 4
Short-Chain Dehydrogenase/Reductase Retsdr8
Tracheobronchial Epithelial Cell-Specific Retinol Dehydrogenase
Rdh-Tbe
Database Links Reactome: R-HSA-2453902
Reactome: R-HSA-5365859
Cellular Localisation Microsome Membrane
Endoplasmic Reticulum Membrane
Associated With Microsomal Membranes
Alternative Antibody Names Anti-Dehydrogenase/Reductase Sdr Family Member 9 antibody
Anti-3-Alpha Hydroxysteroid Dehydrogenase antibody
Anti-3-Alpha-Hsd antibody
Anti-Nadp-Dependent Retinol Dehydrogenase/Reductase antibody
Anti-Rdh-E2 antibody
Anti-Rdhl antibody
Anti-Retinol Dehydrogenase 15 antibody
Anti-Short Chain Dehydrogenase/Reductase Family 9c Member 4 antibody
Anti-Short-Chain Dehydrogenase/Reductase Retsdr8 antibody
Anti-Tracheobronchial Epithelial Cell-Specific Retinol Dehydrogenase antibody
Anti-Rdh-Tbe antibody
Anti-DHRS9 antibody
Anti-RDH15 antibody
Anti-SDR9C4 antibody
Anti-UNQ835 antibody
Anti-PRO1773 antibody

Information sourced from Uniprot.org

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