• Immunofluorescence analysis of NIH/3T3 cells using DGLUCY Rabbit polyclonal antibody (STJ11105233) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using DGLUCY Rabbit polyclonal antibody (STJ11105233) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse heart, using DGLUCY Rabbit polyclonal antibody (STJ11105233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-DGLUCY antibody (269-616) (STJ11105233)

SKU:
STJ11105233

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-D-Glutamate Cyclase-Mitochondrial (269-616) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DGLUCY
Gene ID: 80017
Uniprot ID: GLUCM_HUMAN
Immunogen Region: 269-616
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 269-616 of human DGLUCY (NP_001095836.1).
Immunogen Sequence: ERIPEVHHISQDPLHYSIAS VSASQKIRELESMIGIDPGN RGIGHLLCKDELLKASLSLS HARSVLITTGFPTHFNHEPP EETDGPPGAVALVAFLQALE KEVAIIVDQRAWNLHQKIVE DAVEQGVLKTQIPILTYQGG SVEAAQAFLCKNGDPQTPRF DHLVAIERAGRAADGNYYNA RKMNIKHLVDPIDDLFLAAK KIPGISSTGVGDGGNELGMG KVKEAVRRHIRHGDVIACD
Function D-glutamate cyclase that converts D-glutamate to 5-oxo-D-proline.
Protein Name D-Glutamate Cyclase - Mitochondrial
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-D-Glutamate Cyclase - Mitochondrial antibody
Anti-DGLUCY antibody
Anti-C14orf159 antibody
Anti-UNQ2439 antibody
Anti-PRO5000 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance