• Western blot analysis of lysates from HT-29 cells, using DGKQ Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-DGKQ antibody (691-740 aa) (STJ92702)

SKU:
STJ92702

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Diacylglycerol kinase theta (691-740 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DGKQ
Gene ID: 1609
Uniprot ID: DGKQ_HUMAN
Immunogen Region: 691-740 aa
Specificity: DGK-Theta Polyclonal Antibody detects endogenous levels of DGK-Theta protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DGKQ at the amino acid range 691-740
Function Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription. Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor. Through its diacylglycerol activity also regulates synaptic vesicle endocytosis.
Protein Name Diacylglycerol Kinase Theta
Dag Kinase Theta
Dgktheta
Diglyceride Kinase Theta
Dgk-Theta
Database Links Reactome: R-HSA-114508
Cellular Localisation Cytoplasm
Cytosol
Cell Membrane
Synapse
Cytoskeleton
Nucleus
Nucleus Speckle
Nucleus Matrix
Translocates To The Plasma Membrane In Response To Steroid Hormone Receptor Stimulation
Translocation To The Plasma Membrane Is Dependent On G-Protein Coupled Receptor Stimulation And Subsequent Activation Of Prkce And Probably Prkch
Translocates To The Nucleus In Response To Thrombin Stimulation (Probable)
Association With The Nuclear Matrix Is Regulated By Nerve Growth Factor
Alternative Antibody Names Anti-Diacylglycerol Kinase Theta antibody
Anti-Dag Kinase Theta antibody
Anti-Dgktheta antibody
Anti-Diglyceride Kinase Theta antibody
Anti-Dgk-Theta antibody
Anti-DGKQ antibody
Anti-DAGK4 antibody

Information sourced from Uniprot.org