Anti-DGKH antibody (771-820 aa) (STJ92701)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human DGKH at the amino acid range 771-820
General Information
| Short Description | Rabbit polyclonal anti-Diacylglycerol kinase eta (771-820 aa) for use in IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DGKH |
| Gene ID | 160851 |
| Uniprot ID | DGKH_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human DGKH at the amino acid range 771-820 |
| Immunogen Region | 771-820 aa |
| Specificity | DGK-Eta Polyclonal Antibody detects endogenous levels of DGK-Eta protein. |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation does not inhibit catalytic activity. |
| Function | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization. |
| Protein Name | Diacylglycerol Kinase EtaDag Kinase EtaDiglyceride Kinase EtaDgk-Eta |
| Database Links | Reactome: R-HSA-114508 |
| Cellular Localisation | CytoplasmCell MembraneTranslocated From The Cytoplasm To Endosomes In Response To Stress StimuliIsoform 2 Is Rapidly Relocated Back To The Cytoplasm Upon Removal Of Stress StimuliWhereas Isoform 1 Exhibits Sustained Endosomal AssociationTranslocates From The Cytoplasm To The Cell Membrane In The Presence Of Active Gtp-Bound Form Of Hras |
| Alternative Antibody Names | Anti-Diacylglycerol Kinase Eta antibodyAnti-Dag Kinase Eta antibodyAnti-Diglyceride Kinase Eta antibodyAnti-Dgk-Eta antibodyAnti-DGKH antibody |
Information sourced from Uniprot.org