• Western blot analysis of extracts of various cell lines, using DGAT1 antibody (STJ28938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of mouse liver, using DGAT1 antibody (STJ28938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of MCF7 cells using DGAT1 rabbit polyclonal antibody (STJ28938) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using DGAT1 rabbit polyclonal antibody (STJ28938) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using DGAT1 rabbit polyclonal antibody (STJ28938) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-DGAT1 antibody (263-278) (STJ28938)

SKU:
STJ28938

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DGAT1 (263-278) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DGAT1
Gene ID: 8694
Uniprot ID: DGAT1_HUMAN
Immunogen Region: 263-278
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 263-278 of human DGAT1 (NP_036211.2).
Immunogen Sequence: YELNFPRSPRIRKRFL
Function Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly. In contrast to DGAT2 it is not essential for survival. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases. Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG).
Protein Name Diacylglycerol O-Acyltransferase 1
Acat-Related Gene Product 1
Acyl-Coa Retinol O-Fatty-Acyltransferase
Arat
Retinol O-Fatty-Acyltransferase
Diglyceride Acyltransferase
Database Links Reactome: R-HSA-1482883
Reactome: R-HSA-6798695
Reactome: R-HSA-75109
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Diacylglycerol O-Acyltransferase 1 antibody
Anti-Acat-Related Gene Product 1 antibody
Anti-Acyl-Coa Retinol O-Fatty-Acyltransferase antibody
Anti-Arat antibody
Anti-Retinol O-Fatty-Acyltransferase antibody
Anti-Diglyceride Acyltransferase antibody
Anti-DGAT1 antibody
Anti-AGRP1 antibody
Anti-DGAT antibody

Information sourced from Uniprot.org

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