Anti-Desmoplakin antibody [R06-2V8] (STJA0037121)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Desmoplakin
STJA0037121
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General Information

Short DescriptionRabbit monoclonal anti-Desmoplakin for use in WB, ICC, IF and FC in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ICC/IF/FC
HostRabbit
ReactivityHuman/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR06-2V8
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Purified
Dilution RangeWB 1:500-1:1000
IF 1:50-1:200
FC 1:50-1:100
FormulationRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolDSP
Gene ID1832
Uniprot IDDESP_HUMAN
ImmunogenA synthesized peptide derived from human Desmoplakin

Additional Info

Tissue Specificity Expressed in oral mucosa (at protein level). Expressed in arrector pili muscle (at protein level). Expressed in the heart in the heart (at protein level). Isoform DPI: Apparently an obligate constituent of all desmosomes. Isoform DPII: Resides predominantly in tissues and cells of stratified origin.
Post Translational Modifications Phosphorylation at Ser-2849 increases association with intermediate filament cytokeratin, potentially facilitating interaction between desmosome junctions and intermediate filament architecture.
Function Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance.
Protein Name Desmoplakin
Dp
250/210 Kda Paraneoplastic Pemphigus Antigen
Database Links Reactome: R-HSA-351906
Reactome: R-HSA-6798695
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Reactome: R-HSA-9696264
Reactome: R-HSA-9696273
Cellular Localisation Cell Junction
Desmosome
Cell Membrane
Cytoplasm
Localizes To Desmosome Precursor Particles In The Cytoplasm
Localizes To The Cytoplasm In Undifferentiated Keratinocytes However Becomes Localizes To Both Lateral And Tricellular Cell-Cell Contacts As Differentiation Progresses And As Epithelial Sheet Formation Completes
Alternative Antibody Names Anti-Desmoplakin antibody
Anti-Dp antibody
Anti-250/210 Kda Paraneoplastic Pemphigus Antigen antibody
Anti-DSP antibody

Information sourced from Uniprot.org

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