| Host: | Rabbit |
| Applications: | WB/IHC-F/IHC-P/ICC/IF |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-DENN for use in WB, IHC-F, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R03-8F0 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/mL |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | MADD |
| Gene ID: | 8567 |
| Uniprot ID: | MADD_HUMAN |
| Immunogen: | A synthetic peptide of human DENN |
| Tissue Specificity | Expressed in testis, ovary, brain and heart. Expressed in spleen, thymus, prostate, testis, ovary, small instestine and colon. Expressed in liver. Isoform 1: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. Isoform 2: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. Isoform 3: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. Isoform 4: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. Isoform 5: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. Isoform 6: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. Isoform 7: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. |
| Function | Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family. Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion. Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction. Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses. Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals. Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2. May link TNFRSF1A with MAP kinase activation. May be involved in the regulation of TNFA-induced apoptosis. |
| Protein Name | Map Kinase-Activating Death Domain ProteinDifferentially Expressed In Normal And Neoplastic CellsInsulinoma Glucagonoma Clone 20Rab3 Gdp/Gtp Exchange FactorRabgefRab3 Gdp/Gtp Exchange ProteinRab3gep |
| Database Links | Reactome: R-HSA-5357905Reactome: R-HSA-8876198 Q8WXG6-3 |
| Cellular Localisation | Cell MembraneCytoplasmCell ProjectionAxon |
| Alternative Antibody Names | Anti-Map Kinase-Activating Death Domain Protein antibodyAnti-Differentially Expressed In Normal And Neoplastic Cells antibodyAnti-Insulinoma Glucagonoma Clone 20 antibodyAnti-Rab3 Gdp/Gtp Exchange Factor antibodyAnti-Rabgef antibodyAnti-Rab3 Gdp/Gtp Exchange Protein antibodyAnti-Rab3gep antibodyAnti-MADD antibodyAnti-DENN antibodyAnti-IG20 antibodyAnti-KIAA0358 antibody |
Information sourced from Uniprot.org