| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DEFA5 (20-94) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DEFA5 |
| Gene ID: |
1670 |
| Uniprot ID: |
DEF5_HUMAN |
| Immunogen Region: |
20-94 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-94 of human DEFA5 (NP_066290.1). |
| Immunogen Sequence: |
ESLQERADEATTQKQSGEDN QDLAISFAGNGLSALRTSGS QARATCYCRTGRCATRESLS GVCEISGRLYRLCCR |
| Tissue Specificity | Expressed in the gastrointestinal, reproductive, and urinary tracts (at protein level). Expressed in Paneth cells of the small intestine (at protein level). Expressed throughout the urothelium of the lower urinary tract and in the collecting tubules of the kidney (at protein level). Expressed in stratified squamous epithelial cells of the female genital tract epithelia, such as in vagina, ectocervix, endocervix, endometrium, and fallopian tube (at protein level). Endometrial expression correlates with stages of the menstrual cycle: Expression is low during the early proliferative phase, increased during the mid- to late proliferative phase, peaks during the early secretory phase of the cycle, and decreases during the mid- to late secretory phase. |
| Post Translational Modifications | Glycosylated. Proteolytically cleaved at Arg-62 by trypsin. Both the propeptide form proHD5/HD5(20-94) and HD5(56-94) are cleaved into the lumenal peptide form HD5(63-94) by trypsin. Unprocessed proHD5 exerts antimicrobial activities, but peptide potency is enhanced by peptide processing. Proteolytically cleaved in duodenal fluid.derived fragments are antimicrobially active against commensal bacteria (in vitro). (Microbial infection) The disulfide bridges and homodimerization are a prerequisite for the enhancement of S.flexneri adhesion and invasion. |
| Function | Host-defense peptide that maintains sterility in the urogenital system. Has antimicrobial activity against a wide range of bacteria, including Gram-negative E.coli, P.aeruginosa and S.typhimurium, and Gram-positive E.aerogenes, S.aureus, B.cereus, E.faecium and L.monocytogenes. Confers resistance to intestinal infection by S.typhimurium. Exhibits antimicrobial activity against enteric commensal bacteria such as B.adolescentis, L.acidophilus, B.breve, L.fermentum, B.longum and S.thermophilus. Binds to bacterial membranes and causes membrane disintegration. Induces the secretion of the chemokine IL-8 by intestinal epithelial cells. Binds to B.antracis lef/lethal factor, a major virulence factor from B.anthracis, and neutralizes its enzymatic activity. (Microbial infection) Acts as a target for S.flexneri infection by binding to the bacterium, possibly via bacterial surface proteins, and thereby augmenting infectivity via enhanced bacterial adhesion and invasion of epithelial cells and tissues. |
| Protein Name | Defensin Alpha 5Defensin-5Hd5(20-94 Cleaved Into - Hd5(23-94 - Hd5(29-94 - Hd5(56-94 - Hd5(63-94 |
| Database Links | Reactome: R-HSA-1461973Reactome: R-HSA-1462054 |
| Cellular Localisation | SecretedCytoplasmic VesicleSecretory VesicleStored As Propeptide Hd5(20-94) In Secretory Granules Of Small Intestinal Paneth Cells And Found In The Ileum Lumen As Processed Mature PeptidesPredominantly In The Hd5(63-94) FormPeptides Hd5(20-94)Hd5(23-94) And Hd5(29-94) Are Found Within TissuesHd5(20-94) Being The Predominant Intracellular FormPeptides Hd5(56-94) And Hd5(63-94) Are Found In The Extracellular MilieuHd5(63-94) Being The Most Abundant FormSecreted Into The Female Genital Tract Lumen |
| Alternative Antibody Names | Anti-Defensin Alpha 5 antibodyAnti-Defensin-5 antibodyAnti-Hd5(20-94 Cleaved Into - Hd5(23-94 - Hd5(29-94 - Hd5(56-94 - Hd5(63-94 antibodyAnti-DEFA5 antibodyAnti-DEF5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
