| Function | Involved in nuclear export of spliced and unspliced mRNA. Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. The TREX complex is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway. The THOC1-THOC2-THOC3 core complex alone is sufficient to promote ATPase activity of DDX39B.in the complex THOC2 is the only component that directly interacts with DDX39B. Associates with SARNP/CIP29, which facilitates RNA binding of DDX39B and likely plays a role in mRNA export. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC4 and CHTOP onto mRNA. Also associates with pre-mRNA independent of ALYREF/THOC4. Involved in the nuclear export of intronless mRNA.the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA.its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2.the effect of ALYREF/THOC4 is reported conflictingly with PubMed:23299939 reporting a stimulatory effect. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. |
| Protein Name | Spliceosome Rna Helicase Ddx39b56 Kda U2af65-Associated ProteinAtp-Dependent Rna Helicase P47Dead Box Protein Uap56Hla-B-Associated Transcript 1 Protein |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72163Reactome: R-HSA-72187Reactome: R-HSA-73856Reactome: R-HSA-9013418 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmCan Translocate To The Cytoplasm In The Presence Of Mx1Trex Complex Assembly Seems To Occur In Regions Surrounding Nuclear Speckles Known As Perispeckles |
| Alternative Antibody Names | Anti-Spliceosome Rna Helicase Ddx39b antibodyAnti-56 Kda U2af65-Associated Protein antibodyAnti-Atp-Dependent Rna Helicase P47 antibodyAnti-Dead Box Protein Uap56 antibodyAnti-Hla-B-Associated Transcript 1 Protein antibodyAnti-DDX39B antibodyAnti-BAT1 antibodyAnti-UAP56 antibody |
Information sourced from Uniprot.org
