| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DDIT4 (1-232) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DDIT4 |
| Gene ID: |
54541 |
| Uniprot ID: |
DDIT4_HUMAN |
| Immunogen Region: |
1-232 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1). |
| Immunogen Sequence: |
MPSLWDRFSSSSTSSSPSSL PRTPTPDRPPRSAWGSATRE EGFDRSTSLESSDCESLDSS NSGFGPEEDTAYLDGVSLPD FELLSDPEDEHLCANLMQLL QESLAQARLGSRRPARLLMP SQLVSQVGKELLRLAYSEPC GLRGALLDVCVEQGKSCHSV GQLALDPSLVPTFQLTLVLR LDSRLWPKIQGLFSSANSPF LPGFSQSLTLSTGFRVIKKK LYSSEQLLIEEC |
| Tissue Specificity | Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level). |
| Post Translational Modifications | Phosphorylated by GSK3B.this promotes proteasomal degradation. Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation. |
| Function | Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type.it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes. Required for mTORC1-mediated defense against viral protein synthesis and virus replication. Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death. |
| Protein Name | Dna Damage-Inducible Transcript 4 ProteinHif-1 Responsive Protein Rtp801Protein Regulated In Development And Dna Damage Response 1Redd-1 |
| Database Links | Reactome: R-HSA-5628897 |
| Cellular Localisation | MitochondrionCytoplasmCytosol |
| Alternative Antibody Names | Anti-Dna Damage-Inducible Transcript 4 Protein antibodyAnti-Hif-1 Responsive Protein Rtp801 antibodyAnti-Protein Regulated In Development And Dna Damage Response 1 antibodyAnti-Redd-1 antibodyAnti-DDIT4 antibodyAnti-REDD1 antibodyAnti-RTP801 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
