| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DDIT3 |
| Gene ID: | 1649 |
| Uniprot ID: | DDIT3_HUMAN |
| Immunogen Region: | 1-169 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human DDIT3/CHOP (NP_004074.2). |
| Immunogen Sequence: | MAAESLPFSFGTLSSWELEA WYEDLQEVLSSDENGGTYVS PPGNEEEESKIFTTLDPASL AWLTEEEPEPAEVTSTSQSP HSPDSSQSSLAQEEEEEDQG |
| Post Translational Modifications | Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity. |
| Function | Multifunctional transcription factor in endoplasmic reticulum (ER) stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates.expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress. Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling. |
| Protein Name | Dna Damage-Inducible Transcript 3 Protein Ddit-3 C/Ebp Zeta C/Ebp-Homologous Protein Chop C/Ebp-Homologous Protein 10 Chop-10 Ccaat/Enhancer-Binding Protein Homologous Protein Growth Arrest And Dna Damage-Inducible Protein Gadd153 |
| Database Links | Reactome: R-HSA-380994 Reactome: R-HSA-381183 Reactome: R-HSA-9614657 Reactome: R-HSA-9633012 Reactome: R-HSA-9648895 |
| Cellular Localisation | Cytoplasm Nucleus Present In The Cytoplasm Under Non-Stressed Conditions And Er Stress Leads To Its Nuclear Accumulation |
| Alternative Antibody Names | Anti-Dna Damage-Inducible Transcript 3 Protein antibody Anti-Ddit-3 antibody Anti-C/Ebp Zeta antibody Anti-C/Ebp-Homologous Protein antibody Anti-Chop antibody Anti-C/Ebp-Homologous Protein 10 antibody Anti-Chop-10 antibody Anti-Ccaat/Enhancer-Binding Protein Homologous Protein antibody Anti-Growth Arrest And Dna Damage-Inducible Protein Gadd153 antibody Anti-DDIT3 antibody Anti-CHOP antibody Anti-CHOP10 antibody Anti-GADD153 antibody |
Information sourced from Uniprot.org