Anti-DDDDK-Tag antibody {ABflo® 488} (STJ11109118)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationABflo® 488
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal DDDDK-Tag antibody for use in FC in species independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC |
| Host | Rabbit |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | ABflo® 488 |
| Purification | Affinity purification |
| Dilution Range | FC (intra) , 5 Mu l per 10^6 cells in 100 Mu l volume |
| Formulation | PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3. |
| Storage Instruction | Store at 4°C for up to 1 year from the date of receipt. Do not freeze. |
Target Information
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
Additional Info
| Background | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |
Information sourced from Uniprot.org