| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DDB2 (1-315) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DDB2 |
| Gene ID: |
1643 |
| Uniprot ID: |
DDB2_HUMAN |
| Immunogen Region: |
1-315 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-315 of human DDB2 (NP_000098.1). |
| Immunogen Sequence: |
MAPKKRPETQKTSEIVLRPR NKRSRSPLELEPEAKKLCAK GSGPSRRCDSDCLWVGLAGP QILPPCRSIVRTLHQHKLGR ASWPSVQQGLQQSFLHTLDS YRILQKAAPFDRRATSLAWH PTHPSTVAVGSKGGDIMLWN FGIKDKPTFIKGIGAGGSIT GLKFNPLNTNQFYASSMEGT TRLQDFKGNILRVFASSDTI NIWFCSLDVSASSRMVVTGD NVGNVILLNMDGKELWNLR |
| Tissue Specificity | Ubiquitously expressed.with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed. |
| Post Translational Modifications | Phosphorylation by ABL1 negatively regulate UV-DDB activity. Ubiquitinated by CUL4A in response to UV irradiation. Ubiquitination appears to both impair DNA-binding and promotes ubiquitin-dependent proteolysis. Degradation of DDB2 at sites of DNA damage may be a prerequisite for their recognition by XPC and subsequent repair. CUL4A-mediated degradation appears to be promoted by ABL1. Ubiquitinated, leading to proteasomal degradation, and deubiquitinated by USP24. Deubiquitinated by USP44.leading to its stabilization on DNA lesions. Acetylated. Deacetylation by SIRT6 in response to UV stress facilitates nucleotide excision repair pathway (the NER pathway) transduction. |
| Function | Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR. Isoform D1: Inhibits UV-damaged DNA repair. Isoform D2: Inhibits UV-damaged DNA repair. |
| Protein Name | Dna Damage-Binding Protein 2Ddb P48 SubunitDdbbDamage-Specific Dna-Binding Protein 2Uv-Damaged Dna-Binding Protein 2Uv-Ddb 2 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-5696394Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6796648Reactome: R-HSA-8951664 |
| Cellular Localisation | NucleusChromosomeAccumulates At Sites Of Dna Damage Following Uv Irradiation |
| Alternative Antibody Names | Anti-Dna Damage-Binding Protein 2 antibodyAnti-Ddb P48 Subunit antibodyAnti-Ddbb antibodyAnti-Damage-Specific Dna-Binding Protein 2 antibodyAnti-Uv-Damaged Dna-Binding Protein 2 antibodyAnti-Uv-Ddb 2 antibodyAnti-DDB2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
