| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DCP1A |
| Gene ID: | 55802 |
| Uniprot ID: | DCP1A_HUMAN |
| Immunogen Region: | 303-582 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 303-582 of human DCP1A (NP_060873.4). |
| Immunogen Sequence: | NEKHAPTYTIPLSPVLSPTL PAEAPTAQVPPSLPRNSTMM QAVKTTPRQRSPLLNQPVPE LSHASLIANQSPFRAPLNVT NTAGTSLPSVDLLQKLRLTP QHDQIQTQPLGKGAMVASFS PAAGQLATPESFIEPPSKTA AARVAASASLSNMVLAPLQS MQQNQDPEVFVQPKVLSSAI PVAGAPLVTATTTAVSSVLL APSVFQQTVTRSSDLERKAS SPSPLTIGTPESQRKPSII |
| Tissue Specificity | Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas. |
| Function | Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. Essential for embryonic development. |
| Protein Name | Mrna-Decapping Enzyme 1aSmad4-Interacting Transcriptional Co-ActivatorTranscription Factor Smif |
| Database Links | Reactome: R-HSA-430039Reactome: R-HSA-450385Reactome: R-HSA-450513Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmP-BodyNucleusCo-Localizes With Nanos3 In The Processing BodiesPredominantly CytoplasmicIn Processing Bodies (Pb)NuclearAfter Tgfb1 TreatmentTranslocation To The Nucleus Depends On Interaction With Smad4 |
| Alternative Antibody Names | Anti-Mrna-Decapping Enzyme 1a antibodyAnti-Smad4-Interacting Transcriptional Co-Activator antibodyAnti-Transcription Factor Smif antibodyAnti-DCP1A antibodyAnti-SMIF antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) HeLa (KO) cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ114797) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/93884/375089/STJ114797_1__72218.1713141770.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) HeLa (KO) cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ114797) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) 293T cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ11100746) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89684/358175/STJ11100746_1__66574.1713122483.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FKBP10 knockout (KO) 293T cells, using [KO Validated] FKBP10 Rabbit polyclonal antibody (STJ11100746) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.")
